Journal: Cell Reports Medicine

Article Title: Weight-loss maintenance is accompanied by interconnected alterations in circulating FGF21-adiponectin-leptin and bioactive sphingolipids

doi: 10.1016/j.xcrm.2024.101629

Figure Lengend Snippet: Circulating adiponectin and leptin profiles are ameliorated following weight-loss maintenance with exercise, GLP-1RA, and their combined treatment Levels of and changes in serum adiponectin (A) and leptin (B) as measured by ELISA and radioimmunoassay (RIA), respectively, in fasting blood samples. Time-course data are presented as estimated means ±95% confidence limits. Fold changes are expressed as log 2 fold change relative to baseline (week − 8) and are presented as observed individual values with estimated means ±95% confidence limits. Constrained linear mixed models were used to estimate within- and between-treatment differences. ∗Significant within-treatment change ( p < 0.05).

Article Snippet: Human Leptin RIA Kit , Millipore , HL-81K.

Techniques: Enzyme-linked Immunosorbent Assay, RIA Assay

Journal: Cell Reports Medicine

Article Title: Weight-loss maintenance is accompanied by interconnected alterations in circulating FGF21-adiponectin-leptin and bioactive sphingolipids

doi: 10.1016/j.xcrm.2024.101629

Figure Lengend Snippet: Differential alterations in FGF21, adiponectin, leptin, and individual sphingolipid species in weight maintainers and weight regainers (A) Schematics of the weight “maintainers” and “regainers” subgroup analysis. (B–E) Levels of and changes in circulating metabokines in weight maintainers and weight regainers. Time-course data are presented as estimated means ±95% confidence limits. Changes were computed as log 2 -transformed fold changes (Log 2 FC) at the end of treatment (week 52) relative to baseline (week − 8) and are presented as observed individual values with estimated means ±95% confidence limits. (F) Changes in circulating sphingolipids in weight maintainers and weight regainers. Changes were computed as log 2 -transformed fold changes (Log 2 FC) at the end of treatment (week 52) relative to baseline (week − 8). Linear mixed models were used to estimate within-group changes and between-group differences. ∗Significant within-group change ( p < 0.05). # Significant between-group difference ( p < 0.05).

Article Snippet: Human Leptin RIA Kit , Millipore , HL-81K.

Techniques: Transformation Assay

Journal: Cell Reports Medicine

Article Title: Weight-loss maintenance is accompanied by interconnected alterations in circulating FGF21-adiponectin-leptin and bioactive sphingolipids

doi: 10.1016/j.xcrm.2024.101629

Figure Lengend Snippet:

Article Snippet: Human Leptin RIA Kit , Millipore , HL-81K.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Software, Solvent, Mass Spectrometry

Journal: Archives of Endocrinology and Metabolism

Article Title: Association between plasma leptin/adiponectin ratio and insulin resistance indexes in prepubertal children

doi: 10.20945/2359-4292-2022-0353

Figure Lengend Snippet: Anthropometric and plasma biochemical measurements of Chilean prepubertal children

Article Snippet: Plasma leptin was also measured by RIA (Human Leptin RIA Kit; Linco Research, Inc.; sensitivity 1.0 ng/mL, intra-assay CV 4.98, inter-assay CV 4.5).

Techniques: Clinical Proteomics

Journal: Archives of Endocrinology and Metabolism

Article Title: Association between plasma leptin/adiponectin ratio and insulin resistance indexes in prepubertal children

doi: 10.20945/2359-4292-2022-0353

Figure Lengend Snippet: Plasma leptin, adiponectin, and leptin/adiponectin ratio (LAR) according to body mass index (BMI) categories in prepubertal Chilean children. Linear regression analysis of the association between plasma leptin, plasma adiponectin, and LAR with BMI z-score categories adjusted for age in girls ( A , B , and C ) and boys ( D , E , and F ). *** P value < 0.0001.

Article Snippet: Plasma leptin was also measured by RIA (Human Leptin RIA Kit; Linco Research, Inc.; sensitivity 1.0 ng/mL, intra-assay CV 4.98, inter-assay CV 4.5).

Techniques: Clinical Proteomics

Journal: Archives of Endocrinology and Metabolism

Article Title: Association between plasma leptin/adiponectin ratio and insulin resistance indexes in prepubertal children

doi: 10.20945/2359-4292-2022-0353

Figure Lengend Snippet: Odds ratios for the association of plasma leptin, plasma adiponectin, and leptin/adiponectin ratio with insulin resistance in Chilean prepubertal children

Article Snippet: Plasma leptin was also measured by RIA (Human Leptin RIA Kit; Linco Research, Inc.; sensitivity 1.0 ng/mL, intra-assay CV 4.98, inter-assay CV 4.5).

Techniques: Clinical Proteomics

Journal: Archives of Endocrinology and Metabolism

Article Title: Association between plasma leptin/adiponectin ratio and insulin resistance indexes in prepubertal children

doi: 10.20945/2359-4292-2022-0353

Figure Lengend Snippet: Receiver operating characteristic (ROC) curve analysis to discriminate insulin sensitivity versus resistance among 465 girls (left) and 503 boys (right) using the index triglyceride-glucose corrected for waist circumference (TyG-WC) as a binary classification variable. The TyG-WC 75th percentile was used to identify children with insulin resistance from those with insulin sensitivity. Plasma leptin, plasma adiponectin, leptin/adiponectin ratio (LAR), and BMI z-score were assessed using their areas under the ROC curve.

Article Snippet: Plasma leptin was also measured by RIA (Human Leptin RIA Kit; Linco Research, Inc.; sensitivity 1.0 ng/mL, intra-assay CV 4.98, inter-assay CV 4.5).

Techniques: Clinical Proteomics

Journal: Archives of Endocrinology and Metabolism

Article Title: Association between plasma leptin/adiponectin ratio and insulin resistance indexes in prepubertal children

doi: 10.20945/2359-4292-2022-0353

Figure Lengend Snippet: Receiver operating characteristic (ROC) curve analysis to discriminate insulin sensitivity versus resistance among 465 girls (left) and 503 boys (right) using the homeostasis model assessment of insulin resistance (HOMA-IR) as a binary classification variable. The HOMA-IR 75th percentile was used to identify children with insulin resistance from those with insulin sensitivity. Plasma leptin, plasma adiponectin, leptin/adiponectin ratio (LAR), and body mass index (BMI) z-score were assessed using their areas under the ROC curve.

Article Snippet: Plasma leptin was also measured by RIA (Human Leptin RIA Kit; Linco Research, Inc.; sensitivity 1.0 ng/mL, intra-assay CV 4.98, inter-assay CV 4.5).

Techniques: Clinical Proteomics